This kit is based on the fluorescence quantitative PCR method and designs primer probes targeting the conserved sequences of mycobacteria.withIt is used for qualitative detection of mycobacterial DNA in intermediate products, semi-finished products and finished products of various biological products and drugs.
This kit, when used in conjunction with the Baorui Biological nucleic Acid extraction or Purification Kit, can detect mycobacteria as low as 10CFU in as little as 1.5 hours.
1. Detection limit
The standard strains of 12 common mycobacteria (10CFU) were extracted and detected 24 times respectively, and a total of 24 detection data were obtained. NCS and NTC samples are tested simultaneously in each experiment.
The results show that the NCS and NTC results are qualified. The test results of all 24 10CFU samples are positive, meeting the requirement that at least 23 out of 24 tests have positive results.
Table 1 Summary of detection limit data of 12 standard Mycobacterial strains
Figure 1 shows the amplification diagram of mycobacterium detection
2. Summary of the performance of the reagent kit products
Table 2 Performance Verification Contents and Conclusions of Mycobacterium qPCR Detection Kit
The above data is for reference only. For specific product performance indicators, please refer to the performance verification report of the corresponding item number.
