T7 RNA polymerase 3.0

The by-product dsRNA generated during the IVT reaction strongly stimulates RNA receptors within cells, triggering innate immune signals. BaoruirawThe T7 RNA Polymerase 3.0 independently developed and multi-stage purified has high transcriptional catalytic activity for different templates and different nucleic acid types. Compared with the common T7 RNA Polymerase, the content of the by-product dsRNA produced by transcription of T7 RNA Polymerase 3.0 was significantly reduced. T7 RNA Polymerase 3.0 can precisely recognize the T7 promoter region (5'-TAATACGACTCACTATAG-3') and, starting from G in this region, transcribed the subsequent DNA sequence into single-stranded RNA, using natural nucleotides or modified nucleotides as substrates. Through short-term in vitro transcription incubation, under an appropriate reaction buffer system, users can efficiently obtain a large amount of RNA products from DNA through this enzyme.

The solution is clear and transparent, with no visible foreign substances.
There is no activity of exonuclease or endonuclease.
Protein purity ≥95%.
No DNase or RNase activity.