rcAAV-5/N Detection Kit (PCR-Fluorescence Probe Method)

Adeno-associated virus (AAV) is a widely used gene therapy vector at present and belongs to the parvovirus family.rAAV is a base modified from the wild-type AAVDue to the carrier.rcAAV is on rAAV carriersrawDuring the production process, it developsrawNon-homologous recombination has formed AAV with replication ability, namely the replicating adeno-associated virus. National MedicineThe Quality Supervision and Administration Bureau pointed out that quality control of rcAAV is necessary in gene therapy products.The RCAAV-5 /N detection kit (PCR-fluorescence probe method) can detect the contamination rate of rcAAV in RCAAV-5 /N (where N represents different capsid serotypes).

This kitThe PCR-fluorescence probe method is adopted. The kit contains two systems: the Target system for detecting rcAAV and the Reference system for detecting rAAV. Calibration of two systemsThe product is included in the same quantitative reference product, which can simultaneously achieve rapid, sensitive and specific quantitative detection of rcAAV and rAAV.

This kit, when used in conjunction with the Baorui Biological Nucleic Acid Extraction or Purification Kit I (magnetic bead method), can quantitatively detect rcAAV as low as 20copies/μL within as little as 1.5 hours.

This kit has undergone comprehensive methodological validation in accordance with relevant regulations, guidelines and other document requirements, and can provide performance validation reports.

Product item number：BP-QN19-100

Product advantages

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