E1B Residual DNA Detection Kit (PCR-Fluorescence Probe Method)

HEK293 is a very common expression vector in cell therapy, gene therapy and biopharmaceuticals, and is widely used in the production of proteins, vaccines, anti-cancer reagents and recombinant adenovirus coatings, etc. The genome of HEK293 cells integrates the genes of adenovirus proteins E1A and E1B, so it is inevitable that impurities such as residual DNA of E1A and E1B will be produced. These impurities not only reduce the effectiveness of viral vector-related drugs, but may also bring about safety issues such as infectivity or tumorigenicity. Therefore, regulatory authorities in various countries have strictly controlled the content of residual DNA in biological products.

This kit is based on the fluorescence quantitative PCR method. It designs primer probes specifically for the E1B gene and pairs them with ready-to-use E1B DNA quantitative reference materials to establish a rapid detection and highly sensitive real-time fluorescence quantitative PCR kit. It is used for the quantitative detection of host cells in biological products, such as HEK293 and 293T cells. A dedicated kit for residual E1B DNA from the source.

This kit, when used in conjunction with the Baorui Biological nucleic Acid extraction or Purification Kit, can accurately quantify trace E1B DNA in samples within as little as 1.5 hours.

This kit has undergone comprehensive methodological validation in accordance with relevant regulations, guidelines and other document requirements, and can provide performance validation reports.

Product item Number: BP-QN04-100

Linear range: 1.42×100 copies/μL-1.42×106copies/μL, amplification efficiency: 100.09%, R2 > 0.99.

2. Graph of the amplification curve