dsRNA Residue Quantitative Detection Kit 2.0

During the process of preparing mRNA using T7 RNA polymerase In Vitro Transcription (IVT), by-products such as double-stranded RNA (dSRNA) are generated. dSRNA not only triggers the body's innate immune response, It may also lead to the degradation of mRNA, so the residual amount of dsRNA is a key quality attribute of mRNA drugs.
This kit employs a double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the content of dsRNA in in vitro transcription systems or synthesized mRNA stock solutions. The microplate is coated with capture antibody to form solid-phase antibody. dsRNA standard and sample to be tested are added to the solid-phase antibody microplate. Then, horseradish peroxidase (HRP) -labeled detection antibody is added to form antibody-antigen-antibody complex. After thorough washing, substrate TMB is added for color development. The chromogenic solution is converted into blue under the catalysis of HRP enzyme and eventually into yellow under the action of acid. The depth of the color is positively correlated with the amount of dsRNA in the sample.