dsRNA Residue Quantitative Detection Kit

This kit employs a double antibody sandwich enzyme-linked immunosorbent assay (sandwich ELISA) to detect the content of double-stranded RNA(dsRNA) in in vitro transcription systems or synthesized mRNA stock solutions. The microplate is coated with capture antibodies to form solid-phase antibodies. Double-stranded RNA(dsRNA) calibrators and samples to be tested are added to the solid-phase antibody microplate. Then, biotinylation detection antibodies are added and incubated to form antibody-antigen-antibody complexes. After washing, streptavidin labeled with horseradish peroxidase (HRP) is added. SA). After thorough washing, the substrate TMB is added for color development. The color development solution is catalyzed by HRP enzyme to turn blue and eventually yellow under the action of acid. The depth of the color is positively correlated with the amount of double-stranded RNA(dsRNA) in the sample. The standard curve range of this kit is 0.047-3ng /mL, and the detection limit is 0.047ng/mL.

Item No. : BP-14-96

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