CircRNA Synthesis kit

Circular RNA (circRNA) is an RNA molecule with a circular structure formed by typical 5'-3' phosphodiester bonds. Compared with linear RNA,circRNA does not contain the 5' terminal cap structure and the 3' terminal poly(A), lacks free terminal ends, and is not easily degraded by exonucidases, making it more stable than linear RNA. At present, the commonly used methods for preparing circular RNA through in vitro transcription mainly involve generating linear RNA in vitro first, and then ligation and inclusion through enzymatic methodsThe childSelf-shear ribozyme cleaving ligation cyclization. Developed by Baorui BiotechnologyoneA simple-to-operate kit for preparing circular RNA. The principle of cyclization in the circular RNA preparation kit is that in GTP and Mg²Under the existing conditions, the circular RNA precursor molecules can be automatically cyclized through diester exchange reactions. The operation of this kit only requiresoneThe first step of incubation can be completed, so the cyclization product does not need to be purified and can be directly used in subsequent experiments.

The cyclization reaction system provided by this kit can be scaled up proportionally without significantly affecting the yield of circular RNA products.

Item No. : BP-13-50

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