DNase Residue Quantitative Detection Kit

Deoxyribonuclease (DNase) is an enzyme capable of hydrolyzing the phosphodiester bonds in DNA molecules. Deoxyribonucidase is similar to ribonucidase (RNase) and is widely present in experimental environments and biological objects. Since DNase can degrade nucleic acids, its presence can cause interference in many experiments. Therefore, it is necessary to determine the presence of DNase. The current methods for detecting DNase include nucleic acid hydrolysis gel electrophoresis, ultraviolet spectrophotometry, high performance liquid chromatography (HPLC), and electrochemical methods, etc. However, they have problems such as long time consumption, inability to accurately quantify, low sensitivity, or being limited by instruments and equipment.

The DNase residue quantitative detection kit independently developed and launched by Baorui Biology can rapidly and quantitatively detect DNase based on the principle of fluorescence probe method, and it has the advantages of high sensitivity and simple operation. Principle of DNase Residue Quantitative Detection Kit The DNase substrate used in this kit is a synthetic DNA oligonucleotide probe, with a VIC Fluorophore, also known as the Donor, at one end and a BHQ1 Quencher, also known as the Acceptor, at the other end. The absorption spectra of these two groups have a certain overlap. When the distance between these two fluorescent groups is appropriate, the fluorescence energy is transferred from the donor to the acceptor, resulting in the attenuation of the fluorescence intensity of the donor's fluorescent molecule itself. When the substrate is cleaved by DNase, the head and tail ends of the DNA substrate are separated, and the two groups are separated. The fluorescence of VIC is no longer quenched by BHQ1, and the fluorescence of VIC can be detected. The increase rate of the fluorescence signal is positively correlated with the quantity and activity of the enzyme。

Item No. : BP-16-100

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