2×Fast Direct RT Premix-UNG Ⅳ (Probe qRT-PCR)

Fast DirectRTPremix-UNG Ⅳ (Probe qRT-PCR) It is specifically designed to eliminate the nucleic acid extraction and purification steps of samples and directly conduct fluorescence quantification of target genes.TaqMThe an probe method is a dedicated reagent for RNA amplification detection, containing rapidly amplifying reverse transcriptases and DNA polymerases that have been genetically modified and screened, with an amplification rate of 20 to 40 The PCR reaction is completed within minutes. This product has a strong tolerance to inhibitors and can be used without nucleic acid extraction and purificationThroat swabAnticoagulated whole blood, plasma, serum and other samplesDirect RNA amplification detection.

This reagent is formulated with an optimized formulaThe qPCR-specific Buffer and UNG/dUTP anti-contamination system can effectively prevent false positive amplification caused by residual PCR products and aerosol contamination.

1. 10×Fast Direct RTase/UNG Mix Ⅳ

2. 2×Fast Direct RT Premix Buffer Ⅳ (dUTP)

Reverse transcriptase: 42℃ to 55℃.

2. Antibody-modified Taq, 95℃, 1-5 minutes of hot start.

3. It has strong tolerance to inhibitors from sources such as plasma and swabs.

4. The amplification rate is no less than 1kb/10s, suitable for rapid fluorescence quantitative PCR for purifying RNA, and the detection can be completed within 30 minutes at the fastest.

It is applicable to multiplex and multi-channel QPCR, such as the detection of four different targets in four channels.

2. Suitable for sensitivity amplification of low-concentration RNA templates.

3. Applicable to direct expansion reactions.

4. Rapid amplification detection.

Immediate amplification comparison experiment