SensiDirect RT Premix-UNG (Probe qRT-PCR) (014429) is a dedicated reagent specifically designed to bypass the nucleic acid extraction and purification steps of the sample and directly conduct one-step RT-PCR fluorescence quantification (probe method) detection of target genes. This product has a strong tolerance to inhibitors and can be used for one-step RT-PCR direct amplification detection of anticoagulated whole blood, plasma, serum, throat swabs, saliva and other samples without nucleic acid extraction and purification.
This reagent uses TS-UNG enzyme and an optimized Buffer system containing dUTP, which can effectively prevent false positive reactions caused by residual PCR products and aerosol contamination.
2.5×SensiDirect RT Premix-UNG (Probe qRT-PCR) (014429)
This reagent already contains rapid amplification DNA polymerase, RTase, TS-UNG, PCR Buffer and MgCl2Ingredients such as dNTPs and stabilizers.
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