This kit utilizes T7 RNA polymerase, which can recognize double-stranded DNA templates with the T7 promoter and transcribe DNA sequences downstream of the T7 promoter to efficiently synthesize single-stranded RNA. After in vitro transcription reaction and annealing, dsRNA can be synthesized, and then enzymatic digestion is carried out to remove excess trace impurities (such as single-stranded RNA). Under normal circumstances, a single reaction using this kit can synthesize 15-60μg of dsRNA, with a purity of over 85%.
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