DNaseⅠ

DNase I (Deoxyribonuclease I), also known as deoxyribonuclease I, is an enzyme that can efficiently digest single-stranded or double-stranded DNA fragments. The activity of DNaseⅠ is highly dependent on Ca²+ level and can be Mg²+ or Mn²"Activate." At Mg²Under the existing conditions, DNaseⅠ can randomly cut DNA fragments from any site. In Mn²Under existing conditions, DNaseⅠIt can be cleaved at approximately the same sites of double-stranded DNA to form a flat-end DNA fragment or a sticky end containing 1-2 nucleotide protrusions. When the working pH range is between 7 and 8, DNaseⅠ exhibits the best endonuclease activity and can be applied to remove single-stranded or double-stranded DNA fragments from various RNA samples.

Item Number:BP-E04

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