2×SensiDirect® RT Premix-UNG Ⅳ (Probe qRT-PCR)

SensiDirectRTPremix-UNG Ⅳ (Probe qRT-PCR) It is specifically designed to eliminate the nucleic acid extraction and purification steps of samples and proceed directlyOne-step methodOne Step）RT-PCRSpecial reagents for fluorescence quantitative (probe method) detection of target genesIt contains a rapidly amplifying reverse transcriptase that has been genetically modified and screenedDNA polymerase can complete the PCR reaction within 20 to 40 minutes. This product has a strong tolerance to inhibitors and no further treatment is requiredDNA extraction and purification can be used for anticoagulation of whole blood, plasma and serumThroat swab, salivaSuch as samplesOne-step methodRT-PCRDirect amplification detection.

This reagent adoptsThe TS-UNG enzyme and the optimized Buffer system containing dUTP can effectively prevent false positive reactions caused by residual PCR products and aerosol contamination.

1. 10×SensiDirect RTase/UNG Mix Ⅳ

2. 2×SensiDirect RT Premix Buffer Ⅳ (dUTP)

It has excellent tolerance to inhibitors from samples such as serum and saliva, with saliva ≤2μL and serum ≤30%.

2. It is suitable for rapid fluorescent quantitative RT PCR without extraction, and the detection can be completed within 40 minutes at the fastest.

Detection of RNA viruses related to humans and animals.

2. Direct and rapid amplification detection without extraction.