Protease Inhibitor Cocktail(100×)

Samples such as cell or tissue extracts contain many endogenous proteases, phosphatases, etc., which can easily lead to protein degradation or demodification in the extracts, thereby affecting subsequent protein detection. Therefore, adding appropriate inhibitors of proteases, phosphatases, etc. to extracts and other samples is an effective method to prevent protein degradation and demodification.
This product is Protease Inhibitor Cocktail The EDTA-Free (100×) protease inhibitor mixture contains a broad specificity for aspartic acid protease, cysteine protease, serine protease, acid protease and aminopeptidase Components such as AEBSF, Aprotinin, Bestatin, E-64(trans-Epoxysuccinyl-L-leucylamido -(4-guanidino) butane), Leupeptin and Pepstatin A.
Among them, AEBSF is a water-soluble irreversible inhibitor of serine protease, and its inhibition constant is similar to that of PMSF and DFP. It can effectively inhibit proteases such as trypsin, chymotrypsin, plasmin, kallikrein and thrombin, and serve as a substitute for PMSF and DFP. AEBSF has lower toxicity, better water solubility and stability in aqueous solution. Aprotinin is a competitive reversible inhibitor of serine proteases; Bestatin is a reversible inhibitor of aminopeptidase. E-64 is an irreversible inhibitor of cysteine protease. Leupeptin is a reversible inhibitor of serine and cysteine proteases; Pepstatin A is a reversible inhibitor of aspartic acid protease.

It contains inhibitors of aspartic acid protease, cysteine protease, serine protease, acid protease and aminopeptidase.

Inhibition of proteases in cell or tissue extracts.