Protease and Phosphatase Inhibitor Cocktail，EDTA-Free（100×）

Samples such as cell or tissue extracts contain many endogenous proteases, phosphatases, etc., which can easily lead to protein degradation or demodification in the extracts, thereby affecting subsequent protein detection. Therefore, adding appropriate inhibitors of proteases, phosphatases, etc. to extracts and other samples is an effective method to prevent protein degradation and demodification.
This product, Protease and Phosphatase Inhibitor Cocktail, EDTA-Free (100×) protease and phosphatase inhibitor mixture contains aspartic acid protease, cysteine protease, Serine proteases, acid proteases and aminopeptidases have broad specificity, such as AEBSF, Aprotinin, Bestatin, E-64(trans-Epoxysuccinyl-L-leucylamido-(4-guanidino) butane), and Leupeptin It contains components such as Pepstatin A, sodium fluoride, β-glycerophosphate and sodium orthovanadate phosphatase inhibitors.
Among them, AEBSF is a water-soluble irreversible inhibitor of serine protease, and its inhibition constant is similar to that of PMSF and DFP. It can effectively inhibit proteases such as trypsin, chymotrypsin, plasmin, kallikrein and thrombin, and serve as a substitute for PMSF and DFP. AEBSF has lower toxicity, better water solubility and stability in aqueous solution. Aprotinin is a competitive reversible inhibitor of serine proteases; Bestatin is a reversible inhibitor of aminopeptidase. E-64 is an irreversible inhibitor of cysteine protease. Leupeptin is a reversible inhibitor of serine and cysteine proteases; Pepstatin A is a reversible inhibitor of aspartic acid protease. sodium fluoride is a reversible inhibitor of acid phosphatase. β-glycerophosphate is a reversible inhibitor of serine/threonine phosphatase. sodium orthovanadate is a reversible inhibitor of alkaline phosphatase and tyrosine phosphatase.

It contains protease and phosphatase inhibitors.

Inhibition of proteases and phosphatases in cell or tissue extracts.