One-Step PAGE Gel Fast Preparation Kit（12.5%）

The One-Step PAGE Gel Fast Preparation Kit (12.5%) is applicable to the TrIS-glycine electrophoresis system. It adopts a premixed formula of upper gel and lower gel, and only requires the addition of the improved coagulant to gel, which is simple and fast. The upper layer of tape provided is red, and the sampling holes are clear and easily distinguishable, which is convenient for sampling. Blue concentrated gel can be used to distinguish gels containing different samples. This product comes with an improved accelerator, which has better stability and catalytic efficiency. No additional TEMED needs to be added during the glue mixing process.

One-step potting is simple and fast to operate.
2. Colored upper layer glue.
3. Odorless, no need to use TEMED, avoiding foul smells.
4. The bands are clear, especially the bands of small molecule proteins are clearer than those in traditional gels.

Protein separation (for Western blot/ Western blotting experiments).

2. Monitoring of expression and purification of recombinant proteins and antibodies.

3. Protein purity analysis.

4. Determination of protein molecular weight.

5. Verification of protein-protein interactions (Co-IP, Pull-Down experiments).

Schematic diagram of Maker bands of sublayer collagen at different concentrations:

2. Electrophoresis diagrams of the Maker band of collagen at different concentrations:

Conclusion: At all five gel concentrations, the band edges are clear, the background is clean, and the lanes are neat. Moreover, the bands of each molecular weight of the Marker are well separated, which can meet the full-range separation requirements of proteins from high molecular weight to low molecular weight. This kit offers five commonly used concentrations: 6%, 7.5%, 10%, 12.5% and 15%, enabling proteins of different molecular weights to find ideal separation conditions.