10× Fast Transfer Buffer

The 10×Fast Transfer Buffer is a highly efficient, safe and non-toxic rapid transfer buffer specially designed for Western Blot wet rapid transfer. This product is a 10 times concentrated buffer and needs to be diluted to a 1× concentration with an appropriate amount of deionized water and ethanol before use. This product contains no toxic components. During the preparation process, anhydrous ethanol can be used instead of methanol, avoiding the use of highly toxic reagents such as methanol, making the operation safer and more environmentally friendly. This product is highly efficient and convenient to use. Western Blot wet transfer can be completed within 20 to 40 minutes, and it generates little heat during the transfer process. An internal ice box is sufficient for transfer, eliminating the need for an external ice bath. This product is also applicable to proteins with a wide molecular weight range, effectively solving the problem that large and small proteins cannot be transferred simultaneously on the same membrane. It is compatible with various gels such as the Tris-Gly system, Hepes system, and Bis-Tris system.

1. Environmental protection and safety: Free of toxic components, it is safer and more environmentally friendly to use.

2. Fast and convenient: It only takes 20 to 40 minutes to complete the wet film transfer. During the film transfer process, only the built-in ice packs are needed, and no additional ice baths are required.

3. Wide compatibility: It has excellent transfer effects on proteins within a wide molecular weight range and is fully compatible with various gel systems such as Tris-Glycine, HEPES, and Bis-Tris.

Proteins are transferred from polyacrylamide gel to nitrocellulose (NC) membrane or PVDF membrane through wet electrotransfer (wet transfer).

2. It is suitable for conventional Western Blot experimental procedures that require rapid and efficient membrane transfer.

Baorui Biological Membrane Transfer effect diagram (500mA/20min)

Conclusion: The Baorui biological transfer buffer has good compatibility and can be compatible with both macromolecular and small-molecular-weight proteins.