5×Rapid Blocking Buffer

5×Rapid Blocking Buffer is a blocking solution specially designed for rapid and efficient blocking of transfer membranes. It adopts a protein-free formula and only blocks the transfer membrane without affecting the antibody recognition sites of sample proteins. Therefore, it will not have a shielding effect on the antibody recognition sites of sample proteins, thereby enhancing the specific binding efficiency of antibodies. Compared with the traditional blocking methods using skimmed milk powder or BSA, this product can effectively reduce the non-specific binding of antibodies, significantly reduce background signal interference, ensure the clarity of target signals, and present a higher signal-to-noise ratio in the detection. This product does not contain endogenous phosphorylated proteins or biotin, avoiding interference with the binding of phosphorylated antibodies and biotin detection, and ensuring the accuracy of the results. It has a wide range of applications and is suitable for phosphorylated antibody and biotin detection systems. Highly efficient and convenient, the transfer film can be sealed within 5 to 15 minutes. This product is a 5× concentrated solution and needs to be diluted to a 1× working solution before use.

1. Fast and convenient: It only takes 5 to 15 minutes to complete the membrane sealing.

2. Reliable performance: Compared with traditional skimmed milk powder or BSA blocking methods, it can effectively reduce the non-specific binding of antibodies, significantly reduce background signal interference, ensure clear target signals, and present a higher signal-to-noise ratio in detection.

3. Wide application range: It is suitable for phosphorylation-specific antibodies and biotin-based detection systems.

1. Rapid sealing: Rapidly seal the non-specific binding sites on nitrocellulose (NC) membranes or PVDF membranes.

2. Optimize detection effect: Optimize the signal-to-noise ratio in conventional Western Blot experiments.

3. Suitable for high-sensitivity detection: including downstream detection experiments with high sensitivity requirements such as phosphorylated protein detection and biotinylated protein detection.