5× Rapid sealing liquid

5×Rapid Blocking Buffer is a blocking solution specially designed for rapid and efficient blocking of transfer membranes. It adopts a protein-free formula and only blocks the transfer membrane without affecting the antibody recognition sites of sample proteins. Therefore, it will not have a shielding effect on the antibody recognition sites of sample proteins, thereby enhancing the specific binding efficiency of antibodies. Compared with the traditional blocking methods using skimmed milk powder or BSA, this product can effectively reduce the non-specific binding of antibodies, significantly reduce background signal interference, ensure the clarity of target signals, and present a higher signal-to-noise ratio in the detection. This product does not contain endogenous phosphorylated proteins or biotin, avoiding interference with the binding of phosphorylated antibodies and biotin detection, and ensuring the accuracy of the results. It has a wide range of applications and is suitable for phosphorylated antibody and biotin detection systems. Highly efficient and convenient, the transfer film can be sealed within 5 to 15 minutes. This product is a 5× concentrated solution and needs to be diluted to a 1× working solution before use.

1. High efficiency and convenience: The transfer film can be sealed within 5 to 15 minutes.
2. Reliable performance: Compared with the traditional closed methods using skimmed milk powder or BSA, this product can present a higher signal-to-noise ratio in the detection.

3. Wide application range: Suitable for phosphorylated antibody and biotin detection systems.

It is suitable for closed operations in Western blotting (WB), immunohistochemistry (IHC) and immunofluorescence (IF) experiments.