10×TBST Buffer

10×TBST Buffer is a widely used isotropic buffer salt solution in biological experiments. It is mainly used in Western Blot experiments to wash away non-specifically bound antibodies and other reagents on the membrane, thereby reducing the background and enhancing the signal-to-noise ratio. Its core components include Tris-HCl (pH7.5±0.1) (providing a stable pH buffer system), NaCl (maintaining isotonic conditions), and Tween-20 (acting as a detergent to enhance elution capacity).
This product is suitable for Immunofluorescence (IF), Immunohistochemistry (IHC) and Immunocytochemistry. In experiments such as IC), the preparation of blocking solutions, the dilution of primary or secondary antibodies, and the washing after incubation. This product is a 10× concentrated solution and needs to be diluted to a 1× working solution before use.

Lower the background and enhance the signal-to-noise ratio.
2. Applicable to all antibody dilutions (IF, IHC, IC, ELISA, WB).

3. It does not contain animal-derived proteins such as BSA and skimmed milk powder.

In the Western Blot experiment, wash off the non-specifically bound antibodies and other reagents on the membrane.
2. The preparation of blocking solutions, dilution of primary or secondary antibodies, and washing after incubation in experiments such as immunofluorescence, immunohistochemistry, and immunocytochemistry.