10×TBST Buffer

10×TBST Buffer is a widely used isotropic buffer salt solution in biological experiments. It is mainly used in Western Blot experiments to wash away non-specifically bound antibodies and other reagents on the membrane, thereby reducing the background and enhancing the signal-to-noise ratio. Its core components include Tris-HCl (pH7.5±0.1) (providing a stable pH buffer system), NaCl (maintaining isotonic conditions), and Tween-20 (acting as a detergent to enhance elution capacity).
This product is suitable for Immunofluorescence (IF), Immunohistochemistry (IHC) and Immunocytochemistry. In experiments such as IC), the preparation of blocking solutions, the dilution of primary or secondary antibodies, and the washing after incubation. This product is a 10× concentrated solution and needs to be diluted to a 1× working solution before use.

1. Low background and high signal-to-noise ratio: Effectively reduce non-specific background and improve the detection signal and signal-to-noise ratio.

2. Wide application: It has a wide range of uses and is suitable for diluting various antibodies in multiple experiments such as IF, IHC, ICC, ELISA and WB.

3. No animal protein: Special formula design, free of animal protein components such as bovine serum albumin (BSA) and skimmed milk powder.

It is used for the washing step after antibody incubation in experiments such as Western Blot (WB), immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC).

2. It is used for preparing blocking solutions.

3. It is used for the dilution of primary and secondary antibodies.