T4 DNA Ligase

T4 DNA LigaseCatalyzabledsDNAThe 5 'phosphate terminal and 3' of adjacent nucleic acids at the flat terminal or sticky terminalA phosphodiester bond is formed at the end of the hydroxyl group. This catalytic reaction requiresATPAs an auxiliary factor. Double chains can also be repairedDNA"Double"chainRNAorDNA/RNASingle-strand notches on hybrids, but they cannot catalyze the connection of all-single-stranded nucleotides. Applicable to twoSequencing generationNGSJoint connection in library constructionDNAThe connection of fragments with vectors and others require double-stranded coresExperiments on acid connection, etc.

Double-stranded DNA fragment connection

2.NGS connector connection

3.Gene cloning TA connection