T4 DNA Ligase

T4 DNA Ligase

Efficient connection empowers the entire process of cloning and NGS library construction

This product is an ATP-dependent high-efficiency ligase that can efficiently catalyze the formation of phosphodiester bonds between the 5 '-phosphate terminus and the 3' -hydroxyl terminus of adjacent nucleotides in double-stranded DNA (dsDNA), rapidly repairing the cut in double-stranded molecules. This enzyme can repair single-stranded cuts in double-stranded RNA, DNA/RNA hybrid chains and chimeric nucleic acids, but does not catalyze the ligation of completely single-stranded polynucleotides. Its flat-end ligation efficiency is significantly higher than that of Escherichia coli DNA ligase, and it also supports sticky end ligation, DNA/RNA hybrid strand incision repair and hybrid double-strand gap repair. After strict batch quality control, there is no detectable exonuclease or incision translation activity, making it an ideal choice for various high-sensitivity molecular biology experiments.

1. Broad-spectrum and highly efficient ligation activity
It can efficiently repair incisions in dsDNA, dsRNA, DNA/RNA hybrid chains and chimeric nucleic acids, clearly avoid ligation of completely single-stranded polynucleotides, and prevent non-specific ligation products from interfering with experimental results.

2. Excellent flat end connection performance:
The efficiency of flat-end ligation is significantly higher than that of wild-type Escherichia coli DNA ligase, solving the low efficiency pain points in scenarios such as flat-end cloning and NGS junction ligation.

3. High purity without nuclease contamination
Each batch is verified to have no detectable exonuclease or incision translation activity, avoiding non-specific degradation of nucleic acid substrates and ensuring the reproducibility of experimental results.

4. Friendly reaction conditions:
It is compatible with the conventional connection buffer system and response conditions, and stable results can be obtained in most application scenarios without complex optimization.

1.NGS library construction: Connector connection of amplicon libraries, shotgun libraries, and targeted enrichment libraries; Repair of double-strand gaps in hybrid nucleic acids.

2. Gene cloning: Gene insertion, gene assembly, and site-directed mutagenesis of flat-terminal/viscosity-terminal vectors.

3. Molecular biology detection: Ligation-mediated PCR (LM-PCR), ChIP-seq/ATAC-seq linker ligation, RNA secondary structure analysis.

4. Rna-related experiments: Incision repair of dsRNA/DNA-RNA hybrid strands in RNA-seq, Ribo-seq, in vitro translation and other experiments.

5. Diagnostic reagent research and development: Development of core enzyme raw materials for hybrid and ligation-dependent molecular detection reagents.