Neoscript® One Step RT-PCR Kit

AcuGenix™ LongR One-Step RT-PCR Kit

High-fidelity closed tube systems empower efficient amplification of long RNA templates

This kit is an advanced one-step reverse transcription-PCR (RT-PCR) solution optimized for long RNA templates. Through a combination of directionally evolved co-engineered long fragment enzymes and a buffer system pre-optimized for long amplon synthesis, reverse transcription and high-fidelity PCR are integrated into a single-tube closed reaction without the need for intermediate purification or cap opening operations. Significantly reduce the risk of aerosol contamination and meet the demands of high-throughput detection and clinical research scenarios.

1. Long fragment amplification performance
It can stably amplify long RNA targets larger than 10 kb, with an extension rate of up to 20 seconds per thousand bases. The entire process (from reaction preparation to amplification completion) only takes 30 minutes, which is more than twice as efficient as the 60-90 minutes process of conventional long-fragment one-step RT-PCR kits, significantly shortening the experimental cycle.

2. Ultra-high fidelity
Combined with antiantibody blocking hot-start high-fidelity DNA polymerase, it retains the complete 3 '→5' exonuctase checking activity, with a fidelity approximately 50 times that of wild-type Taq DNA polymerase, significantly reducing the base mismatch risk of long amplons and ensuring the reliability of downstream sequencing and cloning results.

3. Synergistic Enhancement of engineered enzymes
Heat-resistant long fragment reverse transcriptase possesses excellent continuous synthesis capacity and RNA secondary structure untwisting activity, enabling efficient synthesis of full-length cDNA from GC-enriched and structurally complex regions, and addressing the industry pain point of easy interruption in reverse transcription of long RNA templates.

4. Excellent amplification specificity and ease of operation
The pre-optimized buffer integrates long fragment amplification enhancers and background inhibitory components, which can effectively suppress primer dimers and non-specific amplification. It enables uniform amplification of long targets without the need for cumbersome primer titration, reducing the cost of methodological development.

5. Excellent batch stability
Each batch undergoes strict quality control, with no detectable RNase, DNase or exogenous nucleic acid contamination. The inter-batch coefficient of variation (CV) of amplification efficiency and specificity is less than 5%, meeting the reagent stability standard. It can ensure the consistency of experimental results from multiple batches and is suitable for scenarios such as cohort studies and high-throughput detection.

1. Whole genome sequencing of long RNA viruses (such as variant analysis of coronaviruses, influenza viruses, arboviruses, etc.).

2. Full-length transcript analysis (mRNA isomer identification, characterization of long non-coding RNA/lncRNA, circular RNA/circRNA).

3. Full-length cDNA synthesis for single-cell RNA sequencing and spatial transcriptome sequencing.

4. Detection of RNA fusion genes and structural variations in long transcripts.

5. Preparation of long-read RNA sequencing (PacBio Iso-Seq, Oxford Nanopore direct RNA sequencing) templates.