AmpHifi HS DNA Polymerase Ⅲ

AmpHifi HS DNA polymerase III

The new generation of hot-start high-fidelity polymerase empowers high-demand PCR and NGS experiments

This product is a new generation of hot-start high-fidelity DNA polymerase that has been engineered through site-directed mutagenesis and domain recombination. Its DNA binding affinity and continuous synthesis capacity have been significantly enhanced, and it has excellent amplification compatibility with high GC templates and trace template input. Verified by standardized mismatch rate detection, its replication fidelity is approximately 150 times that of wild-type Taq DNA polymerase, making it an ideal choice for high-precision molecular biology experiments.

1. New generation engineering performance:
Through targeted protein engineering optimization, it outperforms conventional high-fidelity polymerases in amplification specificity, yield and template compatibility.

2. Excellent DNA binding capacity and continuous synthesis ability:
It has a stronger affinity for template DNA, and a single binding can complete the synthesis of long fragments, reducing the amplification bias of complex templates.

3. Strong compatibility with difficult-to-expand templates
It can stably amplify high GC targets with local GC content as high as 80%, and is compatible with trace template input as low as pg level, without the need for cumbersome condition optimization.

4. Ultra-high fidelity
The fidelity is approximately 150 times that of wild-type Taq DNA polymerase, significantly reducing the amplification error rate and ensuring the reliability of downstream sequencing, cloning and detection results.

5. Convenient and efficient operation
The hot-start design can prevent non-specific amplification at room temperature, support the configuration of reaction systems at room temperature, and achieve stable results without complex optimization.

1. NGS library construction (amplicon libraries, shotgun libraries, targeted enrichment libraries).

2. Low starting amount/trace template PCR (single-cell, cfDNA, FFPE samples, etc.).

3. Amplification with high GC, repetitive sequences or rich secondary structure templates.

4. High-fidelity gene cloning and site-directed mutagenesis.

5. SNP typing and variant detection.

6. Development of diagnostic reagents for scientific research.