AcuGenix™ LongR Super-Fidelity Master Mix

AcuGenix™ LongR Super-Fidelity Master Mix Ultra-High Fidelity Long Fragment PCR Premix

2× ready-to-use premixes empower high-fidelity amplification of ultra-long fragments

This product is a 2× ready-to-use high-fidelity PCR premix, with a core component of genetically engineered hot-start high-fidelity DNA polymerase. This enzyme has an extremely strong DNA binding affinity and continuous synthesis ability, and shows excellent compatibility with both complex templates and partially degraded nucleic acid templates. Its fidelity is approximately 154 times that of wild-type Taq DNA polymerase, making it an ideal choice for high-precision long-fragment PCR experiments.

The premix integrates proprietary extension enhancement factors and specific enhancement components, which can significantly improve the amplification ability, specificity and yield of ultra-long fragments. When using simple templates such as λDNA and plasmids as substrates, targets up to 30 kb in length can be amplified efficiently. When complex templates such as genomic DNA are used as substrates, fragments up to 20 kb in length can be stably amplified. This premix also has excellent tolerance to PCR inhibitors and can directly amplify crude samples such as bacteria, fungi, plant tissues, and whole blood without the need for pre-extraction of nucleic acids.

All core components (hot-start high-fidelity DNA polymerase, dNTP, and optimized buffer) have been premiered. Only primers and templates need to be added to initiate the reaction, significantly reducing pipetting steps, lowering human errors, and enhancing experimental repeatability.

1. Ultra-high fidelity
The fidelity is approximately 154 times that of wild-type Taq DNA polymerase, significantly reducing the amplification error rate and ensuring the reliability of downstream cloning and sequencing results.

2. Long fragment amplification capability:
Simple templates (λDNA, plasmids) can amplify targets up to 30 kb, and complex genomic DNA can amplify fragments up to 20 kb. Their performance is superior to that of conventional high-fidelity PCR premixes.

3. Compatibility of broad-spectrum templates
It can efficiently amplify complex, partially degraded or inhibitor-containing templates. Crude samples do not require cumbersome nucleic acid purification steps, greatly simplifying the experimental process.

4. Easy operation and strong repeatability
The 2× ready-to-use formula reduces pipetting steps and enhances batch consistency. The optional tracer dye version supports direct loading, accelerating result verification.

5. Excellent amplification specificity
The proprietary specific enhancement component can effectively inhibit non-specific amplification and primer dimer formation, ensuring the purity of the amplification product.

1. Gene cluster cloning and BAC library construction.

2. Assembly of ultra-long fragments required for genome completion mapping and structural variation analysis.

3. Direct PCR for crude samples such as bacteria, fungi, plant tissues, and whole blood.

4. Long-fragment PCR for mutation screening and haplotype analysis.

5. Preparation of long-read sequencing (PacBio, Oxford Nanopore) templates.