phi29 DNA Polymerase

phi29 DNA Polymerase is derived from Bacillus subtilis phage phi29, which has a strong strand displacement ability and continuous synthesis ability. A single extension can produce DNA fragments larger than 70Kb. In addition, phi29 DNA Polymerase also possesses an extremely strong 3 '-5' exonuclease activity, and its fidelity is higher than that of the vast majority of current high-fidelity enzymes.
phi29 DNA Polymerase is suitable for in vitro isothermal DNA polymerization reactions that do not rely on thermal cycling, such as in vitro plasmid preparation, whole genome amplification, rolling loop amplification, SNP detection, etc.

1. Possessing strong strand displacement DNA polymerase.
2. Isothermal amplification.

1. Whole-genome amplification.
2. In vitro preparation of plasmids.
3. Isothermal amplification.