AcuGenix™ mRNA Capture Beads

AcuGenix™ mRNA capture magnetic beads

Complete poly(A)+ RNA is directly captured from the crude lysate without rRNA/tRNA removal

This product is A superparamagnetic microbead with surface immobilized Oligo(dT) modification, specifically designed for the efficient and high-purity isolation of intact poly(A)+ RNA (RNA with a 3 'poly(A) tail) from eukaryotic samples. Through proprietary process optimization, the magnetic beads have a customized Oligo(dT) grafting density, which can meet the requirements of broad-spectrum mRNA capture. The average particle size is approximately 1 μm, and it is uniformly dispersed without agglomeration in the aqueous phase buffer system. High magnetic content and excellent magnetic response dynamics characteristics can significantly shorten the operation time.

mRNA capture is achieved through sequence-specific complementary hybridization between surface-immobilized Oligo(dT) and the 3’poly (A) tail of eukaryotic mRNA. This product can not only efficiently enrich intact poly(A)+ RNA from high-quality total RNA, but also directly capture target RNA from the crude lysates of animal/plant tissues and cultured cells - without the need for pre-removal of rRNA/tRNA, while avoiding the inherent risk of mRNA degradation in the traditional TRIzol extraction process.

Customized Oligo(dT)
Balance high capture efficiency with low non-specific binding, minimize the non-specific enrichment of rRNA, tRNA and fragmented RNA, and ensure the excellent purity of the recovered poly(A)+ RNA.

2. Stable and uniform physical properties:
The average particle size is approximately 1 μm, with no agglomeration phenomenon. The consistency of capture efficiency between batches is excellent. It is suitable for automated liquid handling workstations and has no risk of pipeline blockage.

3. Efficient and time-saving operation
High magnetic content enables rapid magnetic separation (each washing step takes no more than 2 minutes), saving over 50% of manual operation time compared to the columnar mRNA separation method.

4. Flexible adaptation of input samples
It supports two streamlined processes: it can be directly captured from crude tissue/cell lysates (eliminating the RNA extraction step), or purified from pre-isolated total RNA, meeting the needs of different experimental scenarios.

5. Complete preservation of mRNA structure
No contact with organic solvents such as TRIzol is required, avoiding mRNA degradation caused by multi-step purification. The full-length transcript structure is fully preserved, making it suitable for downstream applications such as full-length sequencing.

6. Seamless compatibility across all ecosystems
The recovered mRNA can be directly used in AcuGenix™ 's full range of reverse transcription and NGS library preparation kits, achieving end-to-end process integration without the need for additional optimization.

RNA-seq library construction (conventional transcriptome, single-cell transcriptome, spatial transcriptome).

2. Preparation of full-length cDNA libraries.

3. Gene expression analysis (RT-qPCR, digital PCR).

4. Northern blot, primer extension analysis.

5. Transcriptome studies of low starting amount/easily degradable samples (such as FFPE derived RNA, circulating tumor cells).

6. Targeted mRNA enrichment in customized NGS panels.