AcuGenix™ Universal DNA Library Prep Kit

AcuGenix™ Universal DNA Library Preparation Kit

Dual-platform compatible single-tube integrated process for rapid construction of low initial volume NGS libraries

This kit is a universal dual-index NGS library preparation solution, compatible with all Illumina and MGI high-throughput sequencing platforms. Through innovative design, the three core pre-ligation steps of controllable DNA fragmentation, terminal repair, and 3 '-deoxyadenosine (dA) tail addition are integrated into a single-tube reaction, eliminating the need for intermediate purification between steps. After the reaction, ligation and library enrichment can be directly carried out. The entire manual operation process has been compressed to less than one hour, which can minimize the loss of nucleic acids in precious low-dose samples such as FFPE and cfDNA to the greatest extent.

1. Seamless compatibility across two platforms
It has been strictly verified and can be directly used for cluster generation and on-machine sequencing of the entire series of high-throughput sequencers of Illumina and MGI. In the vast majority of application scenarios, there is no need to adjust the buffer system, and there is no need to develop separate library construction processes for different sequencing platforms.

2. Single-tube integrated process
The three-step reaction is completed in one tube, eliminating the need for three intermediate purification steps. This not only reduces the nucleic acid loss of low initial quantity samples (such as FFPE DNA, cfDNA), but also lowers the risk of aerosol contamination caused by opening the cap.

3. Broad-spectrum sample compatibility
It supports a wide starting amount range of 100 pg - 1 μg of total DNA, and is compatible with various sample types such as animal and plant genomic DNA, microbial genomic DNA, FFPE derived DNA, cell-free DNA (cfDNA), and chromatin immunoprecipitation DNA (ChIP-DNA).

4. Adjustable insertion segments:
The size of the inserted fragments can be precisely controlled by adjusting the fragment reaction time. The default uniform fragment distribution of 150-350 bp is suitable for dual-platform and dual-terminal sequencing requirements, ensuring a balanced coverage of downstream analysis.

5. Stability of high-purity batches
Each batch undergoes strict quality control, with no detectable RNase, DNase or exogenous nucleic acid contamination. The consistency of fragment size distribution between batches is excellent, ensuring the repeatability of sequencing performance.

1. Whole genome sequencing (WGS), whole exome sequencing (WES).

2. Tumor liquid biopsy and other free DNA sequencing (cfDNA-seq).

3. Sequencing of FFPE samples from retrospective clinical studies.

4. Chromatin immunoprecipitation sequencing (ChIP-seq) for epigenetic research.

5. Microbial genome sequencing and metagenomic library construction.