AcuGenix™ Mul-PCR Kit

AcuGenix™ Multiplex PCR Kit (Mul-PCR Kit)

High-throughput targeted amplification features strong uniformity and wide template compatibility

This kit is a high-performance multiplex PCR solution for high-throughput targeted amplification. Its core uses Taq DNA polymerase that has been directionally evolved, significantly enhancing the template affinity. It can efficiently amplify templates that are difficult to handle with conventional polymerases, such as those with low abundance and complex structures.

The kit is equipped with a proprietary and optimized multiplex PCR buffer system, integrating amplification enhancers and background inhibitory components. Compared with traditional multiplex PCR formulas, both amplification efficiency and uniformity of amplification between targets have been significantly improved. It can stably support the amplification of 10 to 600 multiple target panels, is compatible with templates with a wide GC content range of 27% to 80%, and does not require additional adjustment of the buffer system. This formula can effectively reduce the amplification bias between high and low GC targets and ensure the balanced yield of all amplifiers.

The purified amplification products can be directly used in downstream NGS library construction, multiple respiratory pathogen screening, immune library analysis, liquid biopsy targeted enrichment and other scenarios, and are seamlessly compatible with the full range of AcuGenix™ NGS library preparation kits.

1. Engineered high-performance polymerase
The Taq DNA polymerase template modified by directed evolution has stronger affinity and can stably amplify difficult-to-amplify templates with low starting amounts, extreme GC, and complex structures.

2. Excellent multiple amplification performance:
The proprietary buffer system integrates enhancers and inhibitors, offering higher amplification efficiency and better uniformity among targets compared to conventional multiplex PCR reagents.

3. Compatibility between broad-spectrum Panel and template
It can stably support 10 to 600 panels for amplification, is compatible with templates with a wide GC content of 27% to 80%, and does not require additional optimization of the buffer system, significantly reducing the cost of methodology development.

4. Effectively reduce amplification bias
Significantly reduce the yield difference between high and low GC targets, ensure balanced coverage of all amplitons, and improve the accuracy of downstream detection.

Multiple respiratory pathogen screening and infectious disease detection