Klenow Fragment，Exo-

Klenow fragment (3 '→5' exonuclease deficient type)

Directional modification retains 3-end integrity: A dedicated enzyme for NGS library construction and probe labeling

This product is a recombinant mutant of the large fragment of DNA polymerase I in Escherichia coli (E. coli), also known as exonuclease deficient Klenow fragment. The 3 '→5' exonuctase (check) activity of wild-type enzymes was directly inactivated through site-directed mutagenesis technology, while the 5 '→3' DNA polymerase activity was completely retained, and the characteristic of the natural Klenow fragment lacking 5 '→3' exonuctase activity was inherited. This enzyme has no detectable exfoliation degradation activity at the 3 'end of primers or templates, nor significant strand displacement activity. It is an ideal choice for molecular biology experiments that require the preservation of 3' end integrity. After strict batch quality control, it has been confirmed that there are no residues of DNase, RNase or non-specific nucleases, ensuring the repeatability of the experiment.

1. Precise active modification
The targeted inactivation of 3 '→5' exonuclease verification activity fully retains the 5 '→3' polymerization function, without the interference of 5 '→3' exonuclease activity on downstream experiments, and the activity spectrum is clear and controllable.

2.3 End Integrity protection
Avoid non-specific degradation of the 3 'end of primers or templates, and perfectly adapt to experimental scenarios that are sensitive to 3' end modifications.

3. Stability of high-purity batches
Each batch has undergone strict nucleic acid enzyme residue testing, free from DNase, RNase and non-specific nucleic acid enzyme contamination, and the experimental results are highly repeatable.

4. Strong reaction compatibility
It can efficiently catalyze DNA synthesis under mild reaction conditions, is compatible with conventional molecular biology buffer systems and experimental procedures, and does not require complicated condition optimization.

1. NGS library construction: Terminal repair of DNA fragment libraries, synthesis of the second cDNA strand in the RNA-seq process.

2. Nucleic acid probe preparation: Random primer/specific primer labeling (radioactive, fluorescent, biotin labeling).

3. Molecular cloning: Primer extension analysis, site-directed mutagenesis, Sanger dideoxy chain termination sequencing.

4. Genomic research: 5 'protruding end smoothing reaction, preparation of flat-end DNA fragments.

5. Diagnostic reagent development: Development of core enzyme raw materials for amplification or hybridization detection reagents.