Klenow Fragment

Klenow fragment (DNA polymerase I large fragment)

High-fidelity 5 '→3' extranscribed activity adaptive precise molecular experiments

This product, also known as Klenow enzyme, is the C-terminal directed truncation product of E. coli DNA polymerase I. By eliminating redundant domains, the inherent 5 '→3' DNA polymerization activity and 3 '→5' exonucidase proofreading activity of the full-length enzyme are completely retained, while the 5 '→3' exonucidase activity that can lead to non-specific degradation of the template is completely missing. The retained 3 '→5' extranscribed proofreading function can correct mismatched bases in real time during DNA synthesis, providing high-fidelity reaction performance for high-sensitivity molecular experiments.

1. Optimize the extranscribed activity spectrum
Completely remove the non-essential 5 '→3' exonucedal activity to prevent non-specific degradation of template DNA; Retain the powerful 3 '→5' calibration activity to achieve real-time mismatch correction.

2. Fidelity Synthesis
The 3 '→5' extranscribed proofreading activity can efficiently remove mismatched nucleotides, reduce amplification bias and sequence errors, and ensure the reliability of downstream experimental results.

3. High purity and no pollution
Each batch undergoes strict quality control, with no detectable residues of DNase, RNase or non-specific nucleases, meeting the requirements of high-sensitivity experiments.

4. Excellent batch stability
Produced by a controllable recombinant expression system, the performance of each batch is consistent and the experimental results are highly repeatable.

1. NGS library construction: DNA fragment end repair, converting sticky ends into flat ends; The synthesis of the second cDNA strand in the RNA-seq process, with its high-fidelity characteristics, can reduce the sequencing error rate.

2. Nucleic acid probe preparation: Random primer/specific primer extension is used for the preparation of nucleic acid probes labeled with radioactivity, fluorescence, biotin, etc. The absence of 5 '→3' exoglytic activity can prevent the degradation of labeled primers.

3. Molecular cloning: By smoothing out the 5 'protruding end and trimming the 3' protruding end, flat-end DNA is prepared to meet the experimental requirements such as seamless cloning and vector insertion.